Goat anti-Human IgM
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.10356-1mg | €42.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.10356-10mg | €2500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.10356-100mg | €2500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.10356-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.10356-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.10356-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.10356-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.10356 |
|---|---|
| Product Name | Goat anti-Human IgM |
| Isotype | IgG |
| Calculated MW | 950kDa |
| Immunogen | Human IgM purified from human plasma |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.
Protocol / Instructions
The Goat anti-Human IgM antibody is designed for use in various applications including ELISA and Western Blotting.
For ELISA, the recommended dilution is 1:2000-100000. Reagents and buffers required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the plate with the antigen and blocking non-specific binding sites. The antibody is then incubated with the antigen, followed by washing and detection using a conjugated secondary antibody. Technical notes: optimal dilution and incubation times may vary depending on the specific assay conditions.
For Western Blotting, the recommended dilution is 1:1000-20000. Reagents and buffers required include sample buffer, running buffer, transfer buffer, and blocking buffer. Sample preparation involves lysing cells and separating proteins by gel electrophoresis. The antibody is then incubated with the membrane, followed by washing and detection using a conjugated secondary antibody. Technical notes: the antibody may require optimization of dilution and incubation times for optimal results.
Use assay conditions validated for ELISA WB, apply Goat anti-Human IgM at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:2000-100000..
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation times for best results.
Validation Images