Donkey Anti-Goat IgG/Solar Fluor 555
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.10549-100ul | €87.50 |
| Catalog No | CAB.10549 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 555 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 555 is a secondary antibody conjugated to Solar Fluor 555, suitable for various applications including Immunofluorescence (IF).
For IF, the following steps can be followed:
- Reagents or Buffers: Phosphate-buffered saline (PBS), Solar Fluor 555 conjugated secondary antibody, mounting medium with DAPI.
- Sample Preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100 if necessary.
- Antibody Incubation: Incubate samples with primary antibody, followed by the Donkey Anti-Goat IgG/Solar Fluor 555 secondary antibody at a dilution of 1:50-500.
- Washing or Detection: Wash samples thoroughly with PBS, then mount with a medium containing DAPI for nuclear staining.
- Technical Notes: Optimize the dilution of the secondary antibody and the incubation time for the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 555 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 4°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images