Donkey Anti-Guinea Pig IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.12017-50ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.12017-100ul | €100.00 |
| Catalog No | CAB.12017 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/HRP antibody is a versatile tool for various immunological applications. It is essential to follow the recommended dilutions for optimal results.
For Western Blotting, dilute the antibody to 1:1000-10000. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE and transfer them to a membrane. Incubate the membrane with the diluted antibody for 1-2 hours at room temperature. Wash the membrane with a suitable buffer, such as TBS-T, and detect the signal using an enhanced chemiluminescence (ECL) substrate. Technical notes: Ensure proper blocking of the membrane to minimize non-specific binding, and optimize the antibody dilution for the specific application.
For Immunohistochemistry on paraffin sections (IHC-P), dilute the antibody to 1:100-500. Prepare tissue sections by deparaffinization and rehydration. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. Wash the sections with a suitable buffer, and detect the signal using a DAB substrate. Technical notes: Antigen retrieval may be necessary for certain tissues, and the choice of detection system may vary depending on the specific application.
For Immunohistochemistry on frozen sections (IHC-F), dilute the antibody to 1:100-1000. Prepare tissue sections by fixation and permeabilization. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. Wash the sections with a suitable buffer, and detect the signal using a fluorescent secondary antibody. Technical notes: The choice of fixation and permeabilization methods may CABfect the outcome, and the use of a mounting medium with DAPI can help in visualizing nuclei.
For Enzyme-Linked Immunosorbent Assay (ELISA), dilute the antibody to 1:1000-10000. Prepare the antigen by coating it onto a 96-well plate. Incubate the plate with the diluted antibody for 1-2 hours at room temperature. Wash the plate with a suitable buffer, and detect the signal using a TMB substrate. Technical notes: Ensure proper coating and blocking of the plate to minimize non-specific binding, and optimize the antibody dilution for the specific application.
Precautions
Store at 2-8°C, handle with care, include proper controls, and optimize antibody dilution for each application to minimize non-specific binding.
Validation Images