BSA/Solar Fluor 532
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.12390-100ul | €87.50 |
| Catalog No | CAB.12390 |
|---|---|
| Product Name | BSA/Solar Fluor 532 |
| Isotype | N/A |
| Calculated MW | 66kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | BSA |
| Clone Type | N/A |
| Reactivity | BSA |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The BSA/Solar Fluor 532 antibody is recommended for use in Immunofluorescence (IF) applications.
Reagents or buffers required include the BSA/Solar Fluor 532 antibody, a suitable fluorochrome-conjugated secondary antibody, and mounting medium.
Sample preparation involves fixing and permeabilizing cells or tissue sections.
Antibody incubation is performed by applying the primary antibody at a dilution of 1:50-500, followed by incubation for 1-2 hours at room temperature or overnight at 4°C.
Washing is done with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS) to remove unbound primary antibody.
Detection involves applying the secondary antibody and incubating for 1 hour at room temperature, followed by washing and mounting.
Technical notes: Optimize the dilution of the primary antibody and the incubation time to achieve the best signal-to-noise ratio.
Controls should include a negative control without primary antibody to assess non-specific binding.
Use assay conditions validated for IF, apply BSA/Solar Fluor 532 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, optimize antibody dilution and incubation time, include negative controls to ensure specificity.
Validation Images