Mouse Anti-Rabbit IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.12665-100ul | €200.00 |
| Catalog No | CAB.12665 |
|---|---|
| Product Name | Mouse Anti-Rabbit IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Rabbit IgG/APC is a versatile antibody that can be utilized in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers required include the antibody, phosphate-buffered saline (PBS), and a fluorescent mounting medium.
Sample preparation involves fixing and permeabilizing cells or tissue sections.
Antibody incubation is typically performed at a dilution of 1:100-1000 for 1 hour at room temperature.
Washing is done with PBS, and detection involves the use of a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or buffers required include the antibody, PBS, and a flow cytometry buffer.
Sample preparation involves isolating cells and washing them with PBS.
Antibody incubation is typically performed at a dilution of 1:100-1000 for 30 minutes on ice.
Washing is done with PBS, and detection involves the use of a flow cytometer.
Technical notes: Use a proper isotype control to validate the results.
Use assay conditions validated for IF, apply Mouse Anti-Rabbit IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Mouse Anti-Rabbit IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for each application to avoid non-specific binding.
Validation Images