Goat Anti-Guinea Pig IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.13119-100ul | €100.00 |
| Catalog No | CAB.13119 |
|---|---|
| Product Name | Goat Anti-Guinea Pig IgG/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Guinea Pig IgG/HRP is a versatile antibody suitable for various applications. For optimal results, follow the protocols outlined below for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk or BSA for blocking, and a suitable substrate for HRP detection.
Sample preparation: Prepare protein samples and separate them by SDS-PAGE. Transfer the proteins to a membrane.
Antibody incubation: Dilute the antibody in TBST with 1-5% skim milk or BSA, and incubate the membrane for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash the membrane with TBST, and detect the signal using a suitable HRP substrate.
Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions.
Reagents or buffers: Phosphate-buffered saline (PBS), a suitable antigen retrieval solution, and a chromogenic substrate for HRP detection.
Sample preparation: Prepare paraffin-embedded tissue sections, and perform antigen retrieval if necessary.
Antibody incubation: Dilute the antibody in PBS, and incubate the sections for 30-60 minutes at room temperature.
Washing or detection: Wash the sections with PBS, and detect the signal using a suitable chromogenic substrate for HRP.
Technical notes: Optimize the antibody dilution, incubation time, and antigen retrieval method for specific tissue types and experimental conditions.
Reagents or buffers: PBS, a suitable fixative, and a fluorescent substrate or secondary antibody for detection.
Sample preparation: Prepare frozen tissue sections or cells, and fix them with a suitable fixative.
Antibody incubation: Dilute the antibody in PBS, and incubate the samples for 30-60 minutes at room temperature.
Washing or detection: Wash the samples with PBS, and detect the signal using a suitable fluorescent substrate or secondary antibody.
Technical notes: Optimize the antibody dilution, incubation time, and fixation method for specific sample types and experimental conditions.
Reagents or buffers: PBS, a suitable coating buffer, and a chromogenic substrate for HRP detection.
Sample preparation: Prepare antigen-coated plates, and block non-specific binding sites with a suitable blocking buffer.
Antibody incubation: Dilute the antibody in PBS, and incubate the plates for 1-2 hours at room temperature.
Washing or detection: Wash the plates with PBS, and detect the signal using a suitable chromogenic substrate for HRP.
Technical notes: Optimize the antibody dilution, incubation time, and coating conditions for specific experimental conditions.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilutions for specific applications.
Validation Images