Anti-Cadherin 1 Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25ug | CAB.13724-25ug | €302.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.13724-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ug | CAB.13724-100ug | €782.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.13724-100ul | €437.50 |
| Catalog No | CAB.13724 |
|---|---|
| Product Name | Anti-Cadherin 1 Monoclonal Antibody |
| Isotype | IgG1 |
| Calculated MW | N/A |
| Immunogen | Recombinant protein of human Cadherin 1 |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Human |
| Application | WB;IHC-P |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.02% sodium azide. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog，cadherin-6)，R-cadherin，B-cadherin，E/P cadherin and cadherin-5. The classical cadherins，E-，N- and P-cadherin，consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats.
Protocol / Instructions
The Anti-Cadherin 1 Monoclonal Antibody is a mouse-derived antibody that recognizes human Cadherin 1. This antibody has been validated for use in Western Blotting (WB) and Immunohistochemistry-Paraffin (IHC-P) applications.
For Western Blotting, the following reagents and buffers are recommended: RIPA buffer for cell lysis, 10% SDS-PAGE gel for protein separation, and a suitable blocking buffer such as 5% non-fat milk in TBS-T. Sample preparation involves lysing cells in RIPA buffer, separating proteins by SDS-PAGE, and transferring them to a PVDF membrane. The membrane is then blocked and incubated with the Anti-Cadherin 1 Monoclonal Antibody at a suitable dilution. CABter washing, the membrane is incubated with a secondary antibody conjugated to HRP, followed by detection using a chemiluminescent substrate. Technical notes: the optimal antibody dilution and incubation time may vary depending on the specific experimental conditions.
For Immunohistochemistry-Paraffin, the following reagents and buffers are recommended: xylene for deparaffinization, ethanol for rehydration, and a suitable antigen retrieval buffer such as citrate buffer. Sample preparation involves deparaffinizing and rehydrating tissue sections, followed by antigen retrieval. The sections are then blocked and incubated with the Anti-Cadherin 1 Monoclonal Antibody at a suitable dilution. CABter washing, the sections are incubated with a secondary antibody conjugated to a detection system such as DAB or alkaline phosphatase. Technical notes: the optimal antibody dilution and incubation time may vary depending on the specific tissue type and experimental conditions.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images