Donkey Anti-Human IgG/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.14119-100ul | €75.00 |
| Catalog No | CAB.14119 |
|---|---|
| Product Name | Donkey Anti-Human IgG/FITC |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/FITC antibody is a versatile reagent for detecting human IgG in various applications.
For IF, start by preparing your samples, either frozen sections or cultured cells, by fixing them in a suitable fixative such as paraformaldehyde.
Permeabilize the samples if necessary, using a detergent like Triton X-100.
Block non-specific binding sites with a blocking buffer containing serum or BSA.
Incubate the samples with the Donkey Anti-Human IgG/FITC antibody at a dilution of 1:100 to 1:1000 in the blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
Wash the samples thoroughly with a washing buffer like PBS.
Detect the fluorescence using a fluorescence microscope.
Technical notes: The optimal dilution may vary depending on the sample and the experimental setup, so it is recommended to perform a titration to find the best dilution for your specific application.
For FCM, prepare your cell samples by washing them in a suitable buffer like PBS.
If necessary, fix and permeabilize the cells using appropriate reagents.
Block non-specific binding sites with a blocking buffer.
Incubate the cells with the Donkey Anti-Human IgG/FITC antibody at a dilution of 1:100 to 1:1000 in the blocking buffer for 30 minutes to 1 hour on ice.
Wash the cells with a washing buffer.
Analyze the cells using a flow cytometer.
Technical notes: The choice of blocking buffer and the washing steps are critical for reducing background and optimizing the signal-to-noise ratio.
Always include appropriate controls, such as isotype controls or secondary antibody-only controls, to validate the specificity of the staining.
Use assay conditions validated for IF, apply Donkey Anti-Human IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Human IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for each application to ensure specificity and sensitivity.
Validation Images