Goat anti-Chicken IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.14776-100ul | €87.50 |
| Catalog No | CAB.14776 |
|---|---|
| Product Name | Goat anti-Chicken IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Chicken IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Chicken IgG/AP antibody is designed for use in various applications, including Western Blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry on Paraffin sections (IHC-P), and Immunohistochemistry on Frozen sections (IHC-F). The recommended dilution for each application is provided, but optimization may be necessary for specific experimental conditions.
For Western Blotting, dilute the Goat anti-Chicken IgG/AP antibody to 1:1000-10000 in the recommended buffer. Sample preparation involves separating proteins by SDS-PAGE and transferring them to a membrane. Incubate the membrane with the diluted antibody for 1-2 hours at room temperature or overnight at 4°C. Washing is crucial, using a buffer like TBS-T, followed by detection using an appropriate alkaline phosphatase substrate.
In ELISA, the Goat anti-Chicken IgG/AP is used at a dilution of 1:1000-10000. Prepare the antigen by coating the plate with the chicken IgG. Block non-specific binding sites and then incubate with the diluted antibody. Washing steps are essential, followed by the addition of an alkaline phosphatase substrate for detection. Measure the absorbance to determine the presence and amount of the antigen.
For Immunohistochemistry on Paraffin sections, dilute the antibody to 1:100-1000. Sample preparation involves deparaffinization, rehydration, and antigen retrieval. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. Washing is necessary, followed by detection using an alkaline phosphatase substrate. Counterstaining with hematoxylin can help in visualizing the tissue morphology.
In Immunohistochemistry on Frozen sections, the Goat anti-Chicken IgG/AP is used at a dilution of 1:100-1000. Prepare the frozen sections by fixing them in a suitable fixative. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. Washing steps are critical, followed by detection. Counterstaining can be performed to visualize the morphology of the tissue.
Technical notes: Optimization of the antibody dilution and incubation times may be necessary for specific applications and tissues. Controls, including positive and negative controls, should be included to validate the specificity of the staining.
Precautions
Store at -20°C, handle with gloves, include positive and negative controls, and optimize antibody dilution and incubation times for specific applications.
Validation Images