Anti-Acetyl-Histone H3-K18 Polyclonal Antibody

Western Blotting Protocol Reagents or buffers: Anti-Acetyl-Histone H3-K18 Polyclonal Antibody, blocking buffer, primary antibody dilution buffer, secondary antibody, and detection reagents. Sample preparation: Prepare whole cell lysates or nuclear extracts from cells or tissues of interest. Separate proteins by SDS-PAGE and transfer to a membrane. Antibody incubation: Incubate the membrane with the Anti-Acetyl-Histone H3-K18 Polyclonal Antibody at a dilution of 1:500-1000 in primary antibody dilution buffer for 1-2 hours at room temperature or overnight at 4°C. Washing or detection: Wash the membrane with washing buffer and detect using a suitable secondary antibody and detection reagents. Dot Blotting Protocol Reagents or buffers: Anti-Acetyl-Histone H3-K18 Polyclonal Antibody, blocking buffer, primary antibody dilution buffer, secondary antibody, and detection reagents. Sample preparation: Prepare whole cell lysates or nuclear extracts from cells or tissues of interest and apply directly to a membrane. Antibody incubation: Incubate the membrane with the Anti-Acetyl-Histone H3-K18 Polyclonal Antibody at a dilution of 1:500-1000 in primary antibody dilution buffer for 1-2 hours at room temperature or overnight at 4°C. Washing or detection: Wash the membrane with washing buffer and detect using a suitable secondary antibody and detection reagents. IHC-P Protocol Reagents or buffers: Anti-Acetyl-Histone H3-K18 Polyclonal Antibody, blocking buffer, primary antibody dilution buffer, secondary antibody, and detection reagents. Sample preparation: Prepare paraffin-embedded tissue sections and deparaffinize. Antibody incubation: Incubate the sections with the Anti-Acetyl-Histone H3-K18 Polyclonal Antibody at a dilution of 1:50-200 in primary antibody dilution buffer for 1-2 hours at room temperature or overnight at 4°C. Washing or detection: Wash the sections with washing buffer and detect using a suitable secondary antibody and detection reagents. IF/ICC Protocol Reagents or buffers: Anti-Acetyl-Histone H3-K18 Polyclonal Antibody, blocking buffer, primary antibody dilution buffer, secondary antibody, and detection reagents. Sample preparation: Prepare fixed cells or tissue sections. Antibody incubation: Incubate the cells or sections with the Anti-Acetyl-Histone H3-K18 Polyclonal Antibody at a dilution of 1:50-200 in primary antibody dilution buffer for 1-2 hours at room temperature or overnight at 4°C. Washing or detection: Wash the cells or sections with washing buffer and detect using a suitable secondary antibody and detection reagents. ELISA Protocol Reagents or buffers: Anti-Acetyl-Histone H3-K18 Polyclonal Antibody, coating buffer, blocking buffer, primary antibody dilution buffer, secondary antibody, and detection reagents. Sample preparation: Prepare whole cell lysates or nuclear extracts from cells or tissues of interest and coat onto an ELISA plate. Antibody incubation: Incubate the plate with the Anti-Acetyl-Histone H3-K18 Polyclonal Antibody at a dilution of 1:500-1000 in primary antibody dilution buffer for 1-2 hours at room temperature or overnight at 4°C. Washing or detection: Wash the plate with washing buffer and detect using a suitable secondary antibody and detection reagents. ChIP Protocol Reagents or buffers: Anti-Acetyl-Histone H3-K18 Polyclonal Antibody, sonication buffer, IP buffer, and detection reagents. Sample preparation: Prepare cross-linked chromatin from cells or tissues of interest and sonicate to fragment the chromatin. Antibody incubation: Incubate the chromatin with 5ug of the Anti-Acetyl-Histone H3-K18 Polyclonal Antibody in IP buffer for 1-2 hours at room temperature or overnight at 4°C. Washing.