Anti-BrdU Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.15464-50ul | €562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.15464-100ul | €1022.50 |
| Catalog No | CAB.15464 |
|---|---|
| Product Name | Anti-BrdU Monoclonal Antibody |
| Isotype | IgG |
| Calculated MW | N/A |
| Immunogen | Bromodeoxyuridine |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | ALL |
| Application | ELISA;IF/ICC |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.
Protocol / Instructions
The Anti-BrdU Monoclonal Antibody is a valuable tool for detecting bromodeoxyuridine (BrdU) incorporation in various applications. Below are the protocols for the validated applications.
For ELISA, the Anti-BrdU Monoclonal Antibody can be used to detect BrdU incorporation in DNA. Reagents or buffers required include the antibody, BrdU-labeled DNA, and an ELISA plate. Sample preparation involves coating the ELISA plate with BrdU-labeled DNA. Antibody incubation is performed by adding the Anti-BrdU Monoclonal Antibody at the recommended dilution. Washing and detection are carried out according to standard ELISA protocols. Technical notes: Ensure proper blocking and washing steps to minimize non-specific binding.
For Immunofluorescence (IF) or Immunocytochemistry (ICC), the Anti-BrdU Monoclonal Antibody is used to detect BrdU incorporation in cells. Reagents or buffers required include the antibody, a fluorescent secondary antibody, and a mounting medium. Sample preparation involves fixing and permeabilizing cells, followed by denaturation of DNA to expose the BrdU epitope. Antibody incubation is performed by adding the Anti-BrdU Monoclonal Antibody at the recommended dilution. Washing and detection are carried out using a fluorescent secondary antibody. Technical notes: Optimize the denaturation step to achieve the best signal-to-noise ratio.
Precautions
Store at 2-8°C. Handle with care, and avoid freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for each application.
Validation Images