Goat anti-Chicken IgY(IgG) (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.16618-0.5mL | €362.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.16618-1mL | €2625.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.16618-5mL | €2625.00 |
| Catalog No | CAB.16618 |
|---|---|
| Product Name | Goat anti-Chicken IgY(IgG) (Immune serum) |
| Isotype | N/A |
| Calculated MW | 180kDa |
| Immunogen | Chicken IgY full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Chicken |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
In chickens， immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG， it is composed of two light and two heavy chains. Structurally， these two types of immunoglobulin differ primarily in the heavy chains， which in IgY have a molecular mass of about 65，100 atomic mass units (amu)， and are thus larger than in IgG. The light chains in IgY， with a molar mass of about 18，700 amu， are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167，000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally， IgY is partially comparable to Immunoglobulin E (IgE)， as well as to IgG. However， in contrast to IgG， IgY does not bind to Protein A， to Protein G， or to cellular Fc receptors. Furthermore， IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem， CABter they were able to show differences between the immunoglobulins found in chicken eggs， and immunoglobulin G. Other synonymous names are Chicken IgG， Egg Yolk IgG， and 7S-IgG.
Protocol / Instructions
The Goat anti-Chicken IgY(IgG) (Immune serum) is a versatile antibody that can be used in various applications. Below are the protocols for each validated application.
For ELISA, the recommended dilution is 1:5000-400000. The reagents required include the antigen, coating buffer, blocking buffer, and substrate. Prepare the sample by coating the antigen onto the plate, followed by blocking with a suitable blocking buffer. Incubate the antibody at the recommended dilution for 1-2 hours, followed by washing and detection using a suitable enzyme-linked secondary antibody. Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the sample, transfer buffer, and blocking buffer. Prepare the sample by separating the proteins using SDS-PAGE, followed by transfer onto a membrane. Incubate the antibody at the recommended dilution for 1-2 hours, followed by washing and detection using a suitable detection method. Technical notes: Optimize the transfer and blocking conditions to minimize non-specific binding.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the antigen and agarose. Prepare the sample by dissolving the agarose in a suitable buffer, followed by pouring into a petri dish. Create wells in the agarose and add the antigen and antibody. Incubate for 24-48 hours, followed by visualization of the precipitin line. Technical notes: Optimize the concentration of the antigen and antibody to achieve a clear precipitin line.
The Goat anti-Chicken IgY(IgG) (Immune serum) is a valuable tool for detecting Chicken IgY in various applications. By following these protocols, researchers can achieve reliable and reproducible results.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize conditions to minimize non-specific binding.
Validation Images