Donkey Anti-Goat IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.16940-100ul | €87.50 |
| Catalog No | CAB.16940 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/AP antibody is a versatile reagent that can be applied in various immunological assays.
For Western blotting, the antibody should be diluted to 1:1000-10000 in a suitable buffer such as Tris-buffered saline with Tween 20 (TBST).
Sample preparation involves separating proteins by SDS-PAGE and transferring them to a membrane.
The membrane is then incubated with the diluted antibody for 1-2 hours at room temperature or overnight at 4°C.
CABter washing with TBST, the membrane is incubated with a suitable alkaline phosphatase substrate to detect the bound antibody.
Technical notes: Optimize the blocking conditions to minimize non-specific binding.
For Immunohistochemistry on paraffin-embedded tissues (IHC-P), the antibody is diluted to 1:100-1000 in a suitable buffer.
Sample preparation involves deparaffinization, rehydration, and antigen retrieval.
The sections are then incubated with the diluted antibody for 30 minutes to 1 hour at room temperature.
CABter washing with a buffer, the sections are incubated with a suitable alkaline phosphatase substrate to detect the bound antibody.
Technical notes: Use a suitable positive control to validate the staining.
For Immunohistochemistry on frozen tissues (IHC-F), the antibody is diluted to 1:100-1000 in a suitable buffer.
Sample preparation involves fixing and permeabilizing the tissue sections.
The sections are then incubated with the diluted antibody for 30 minutes to 1 hour at room temperature.
CABter washing with a buffer, the sections are incubated with a suitable alkaline phosphatase substrate to detect the bound antibody.
Technical notes: Optimize the fixation conditions to preserve the antigenicity.
For Enzyme-Linked Immunosorbent Assay (ELISA), the antibody is diluted to 1:1000-10000 in a suitable buffer such as carbonate/bicarbonate buffer.
Sample preparation involves coating the plate with the antigen and blocking non-specific binding sites.
The diluted antibody is then added to the wells and incubated for 1-2 hours at room temperature.
CABter washing with a buffer, a suitable alkaline phosphatase substrate is added to detect the bound antibody.
Technical notes: Use a suitable positive control to validate the assay.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize assay conditions to minimize non-specific binding and ensure reproducibility.
Validation Images