Goat anti-Chicken IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1ml | CAB.17096-1ml | €35.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.17096-100ul | €250.00 |
| Catalog No | CAB.17096 |
|---|---|
| Product Name | Goat anti-Chicken IgG/RBITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Chicken IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Chicken IgG/RBITC antibody is designed for use in various immunofluorescence (IF) applications.
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100 for permeabilization if necessary, and a mounting medium with DAPI for nuclear staining.
Sample preparation: Fix cells or tissue sections with a suitable fixative such as paraformaldehyde, followed by permeabilization if required.
Antibody incubation: Dilute the Goat anti-Chicken IgG/RBITC antibody to the recommended dilution of 1:50-500 in PBS, and incubate with the sample for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash the sample thoroughly with PBS, and then proceed with detection using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time based on the specific application and sample type.
Use assay conditions validated for IF, apply Goat anti-Chicken IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions for best results.
Validation Images