Goat Anti-Mouse IgM/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.17647-100ul | €100.00 |
| Catalog No | CAB.17647 |
|---|---|
| Product Name | Goat Anti-Mouse IgM/FITC |
| Isotype | N/A |
| Calculated MW | 900kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Mouse IgM |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Mouse IgM/FITC antibody is a versatile reagent for detecting mouse IgM in various applications.
For IF, start by preparing your samples, either by fixing cells or tissue sections with a suitable fixative such as paraformaldehyde.
Permeabilize the samples if necessary, using a detergent like Triton X-100.
Block non-specific binding sites with a blocking buffer containing bovine serum albumin (BSA) or normal goat serum.
Incubate the samples with the Goat Anti-Mouse IgM/FITC antibody at a dilution of 1:100 to 1:1000 in the blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
Wash the samples thoroughly with a washing buffer like phosphate-buffered saline (PBS) to remove unbound antibody.
Technical notes: The optimal dilution may vary depending on the specific application and the experimental setup.
It is recommended to optimize the antibody dilution for each experiment.
For FCM, prepare your cell suspensions and wash them with a suitable buffer like PBS.
If necessary, fix and permeabilize the cells using appropriate reagents.
Block non-specific binding sites with a blocking buffer.
Incubate the cells with the Goat Anti-Mouse IgM/FITC antibody at a dilution of 1:100 to 1:1000 for 15-30 minutes at 4°C.
Wash the cells with the washing buffer to remove unbound antibody.
Analyze the cells using a flow cytometer with the appropriate settings for FITC detection.
Technical notes: The choice of fixative and permeabilization method may CABfect the antibody binding and the overall performance of the assay.
Always use proper controls to validate the specificity of the staining.
Use assay conditions validated for IF, apply Goat Anti-Mouse IgM/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Mouse IgM/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for each experiment.
Validation Images