Mouse Anti-human IgG(3D3)/Solar Fluor 488
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.17943-100ul | €200.00 |
| Catalog No | CAB.17943 |
|---|---|
| Product Name | Mouse Anti-human IgG(3D3)/Solar Fluor 488 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | human IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-human IgG(3D3)/Solar Fluor 488 antibody is a valuable tool for detecting human IgG in various applications. Below are the protocols for the validated applications.
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and mounting medium.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block non-specific binding sites.
Antibody incubation: Incubate samples with the Mouse Anti-human IgG(3D3)/Solar Fluor 488 antibody at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: Wash samples with PBS and detect using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions.
Reagents or buffers: Phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA), and 0.1% sodium azide.
Sample preparation: Prepare cell suspensions and block non-specific binding sites with 1% BSA.
Antibody incubation: Incubate cells with the Mouse Anti-human IgG(3D3)/Solar Fluor 488 antibody at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: Wash cells with PBS and analyze using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions.
Use assay conditions validated for IF, apply Mouse Anti-human IgG(3D3)/Solar Fluor 488 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Mouse Anti-human IgG(3D3)/Solar Fluor 488 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for specific applications.
Validation Images