Donkey Anti-Human IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.18057-100ul | €200.00 |
| Catalog No | CAB.18057 |
|---|---|
| Product Name | Donkey Anti-Human IgG/AP |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/AP antibody is a versatile tool for detecting human IgG in various applications. Below are the protocols for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and a suitable AP substrate.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a membrane.
Antibody incubation: Incubate the membrane with the Donkey Anti-Human IgG/AP antibody at a dilution of 1:1000-10000 in TBST with 5% skim milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST, then detect using a suitable AP substrate.
Technical notes: Optimize the blocking conditions and antibody dilution to minimize background.
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100, and a suitable AP substrate.
Sample preparation: Fix and embed tissue samples, then section and mount on slides.
Antibody incubation: Incubate the sections with the Donkey Anti-Human IgG/AP antibody at a dilution of 1:100-500 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the sections three times with PBS, then detect using a suitable AP substrate.
Technical notes: Perform antigen retrieval if necessary, and optimize the antibody dilution to achieve the best signal-to-noise ratio.
Reagents or buffers: PBS, Triton X-100, and a suitable fluorescence substrate.
Sample preparation: Fix and permeabilize cells or tissue samples, then mount on slides.
Antibody incubation: Incubate the samples with the Donkey Anti-Human IgG/AP antibody at a dilution of 1:100-1000 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the samples three times with PBS, then detect using a suitable fluorescence substrate.
Technical notes: Optimize the fixation and permeabilization conditions to preserve the antigenicity of the target protein.
Reagents or buffers: Coating buffer, blocking buffer, and a suitable AP substrate.
Sample preparation: Coat the ELISA plate with the antigen, then block with a suitable blocking buffer.
Antibody incubation: Incubate the plate with the Donkey Anti-Human IgG/AP antibody at a dilution of 1:1000-10000 in the blocking buffer for 1 hour at room temperature.
Washing or detection: Wash the plate three times with PBS, then detect using a suitable AP substrate.
Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and reaction conditions to achieve the best results.
Validation Images