Goat anti-Human IgG-Fc
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.18513-1mg | €67.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.18513-10mg | €4167.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.18513-100mg | €4167.50 |
| Catalog No | CAB.18513 |
|---|---|
| Product Name | Goat anti-Human IgG-Fc |
| Isotype | IgG |
| Calculated MW | 52kDa |
| Immunogen | Human IgG-Fc purified from human plasma |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
The Fc region is the constant region on an immunoglobulin molecule，the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.
Protocol / Instructions
The Goat anti-Human IgG-Fc antibody is a versatile tool for detecting human IgG in various applications. Below are the protocols for the validated applications.
Reagents or buffers: Goat anti-Human IgG-Fc antibody, human IgG, ELISA plates, blocking buffer, washing buffer, substrate.
Sample preparation: Coat ELISA plates with human IgG, block with blocking buffer.
Antibody incubation: Incubate with Goat anti-Human IgG-Fc antibody at a dilution of 1:2000-100000.
Washing or detection: Wash with washing buffer, detect with substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Goat anti-Human IgG-Fc antibody, human IgG, SDS-PAGE gel, transfer buffer, blocking buffer, washing buffer.
Sample preparation: Separate human IgG by SDS-PAGE, transfer to a membrane.
Antibody incubation: Incubate with Goat anti-Human IgG-Fc antibody at a dilution of 1:1000-20000.
Washing or detection: Wash with washing buffer, detect with a suitable detection method.
Technical notes: Use a suitable secondary antibody for detection, optimize the antibody dilution and incubation time for best results.
Use assay conditions validated for ELISA WB, apply Goat anti-Human IgG-Fc at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:2000-100000..
Precautions
Store at -20°C, handle with care, use appropriate controls, optimize antibody dilution and incubation time for best results.
Validation Images