Goat anti-Human Fibrinogen (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.18526-0.5mL | €350.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.18526-1mL | €2000.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.18526-5mL | €2000.00 |
| Catalog No | CAB.18526 |
|---|---|
| Product Name | Goat anti-Human Fibrinogen (Immune serum) |
| Isotype | N/A |
| Calculated MW | 95/340kDa |
| Immunogen | Human Fibrinogen |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha， beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains， respectively. In this way fibrinogen is converted into fibrin， which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI， thromboembolism and thromboses of arteries and veins， since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation， tumor growth and many other diseases.This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-Human Fibrinogen (Immune serum) is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-200000. The reagents required include the antibody, antigen, and ELISA kit components. Sample preparation involves coating the plate with the antigen, followed by blocking and washing steps. The antibody is then incubated with the antigen, and CABter washing, the detection reagent is added. Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the antibody, sample buffer, and Western Blotting kit components. Sample preparation involves lysing the cells and separating the proteins by gel electrophoresis. The proteins are then transferred to a membrane, and the antibody is incubated with the membrane. CABter washing, the detection reagent is added. Technical notes: Use a suitable blocking buffer to reduce non-specific binding.
For Double Immunodiffusion, the recommended dilution is 1:8-64. The reagents required include the antibody, antigen, and agarose gel. Sample preparation involves preparing the antigen and antibody solutions, which are then added to the gel. The gel is then incubated, allowing the antibody and antigen to diffuse and form a precipitin line. Technical notes: Optimize the concentration of the antibody and antigen to achieve a clear precipitin line.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for best results.
Validation Images