Donkey Anti-Goat IgG/Solar Fluor 633
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.18542-100ul | €87.50 |
| Catalog No | CAB.18542 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 633 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 633 is a secondary antibody used for detecting goat IgG primary antibodies. It is recommended for use in immunofluorescence (IF) applications.
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and a blocking buffer.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, then permeabilize with 0.1% Triton X-100.
Antibody incubation: Incubate the sample with the primary goat IgG antibody, followed by incubation with the Donkey Anti-Goat IgG/Solar Fluor 633 secondary antibody at a dilution of 1:50-500.
Washing or detection: Wash the sample thoroughly with PBS, then detect the signal using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time to achieve the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 633 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time to minimize background and ensure specific binding.
Validation Images