Donkey Anti-Goat IgG/Solar Fluor 647
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.18728-100ul | €87.50 |
| Catalog No | CAB.18728 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 647 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 647 is a secondary antibody used for detecting goat IgG primary antibodies. It is conjugated to Solar Fluor 647, a fluorescent dye that emits at 665 nm.
For immunofluorescence, the following steps can be followed:
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and a blocking buffer.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde for 15 minutes, then permeabilize with 0.1% Triton X-100 for 10 minutes.
Antibody incubation: Incubate the sample with the primary goat IgG antibody, followed by incubation with the Donkey Anti-Goat IgG/Solar Fluor 647 secondary antibody at a dilution of 1:50-500 for 1 hour at room temperature.
Washing or detection: Wash the sample with PBS three times, then detect the fluorescence using a fluorescence microscope.
Technical notes: Optimize the dilution of the secondary antibody and the incubation time to achieve the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 647 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for best results.
Validation Images