Donkey Anti-Goat IgG/Bio
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.18943-100ul | €27.50 |
| Catalog No | CAB.18943 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Bio |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Bio is a versatile antibody that can be used in various applications. For optimal results, follow the specific protocols outlined below for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), non-fat dry milk, and a suitable substrate for detection.
Sample preparation: Prepare protein samples and separate them using SDS-PAGE. Transfer the proteins to a membrane.
Antibody incubation: Incubate the membrane with the Donkey Anti-Goat IgG/Bio at a dilution of 1:1000-10000 in TBST with 5% non-fat dry milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST and detect using a suitable substrate.
Technical notes: Optimize the dilution and incubation time for best results.
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100, and a suitable chromogen.
Sample preparation: Prepare paraffin-embedded tissue sections and deparaffinize them.
Antibody incubation: Incubate the sections with the Donkey Anti-Goat IgG/Bio at a dilution of 1:100-1000 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the sections with PBS and detect using a suitable chromogen.
Technical notes: Antigen retrieval may be necessary for some tissues.
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100, and a suitable fluorophore.
Sample preparation: Prepare frozen tissue sections and fix them.
Antibody incubation: Incubate the sections with the Donkey Anti-Goat IgG/Bio at a dilution of 1:100-1000 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the sections with PBS and detect using a suitable fluorophore.
Technical notes: Use a mounting medium with an anti-fade agent to preserve fluorescence.
Reagents or buffers: Coating buffer, blocking buffer, and a suitable substrate.
Sample preparation: Coat the plate with the antigen and block with a suitable blocking buffer.
Antibody incubation: Incubate the plate with the Donkey Anti-Goat IgG/Bio at a dilution of 1:1000-10000 in the blocking buffer for 1 hour at room temperature.
Washing or detection: Wash the plate and detect using a suitable substrate.
Technical notes: Optimize the coating and blocking conditions for best results.
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100, and a suitable fluorophore.
Sample preparation: Prepare cell samples and fix them.
Antibody incubation: Incubate the cells with the Donkey Anti-Goat IgG/Bio at a dilution of 1:100-1000 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the cells with PBS and detect using a suitable fluorophore.
Technical notes: Use a mounting medium with an anti-fade agent to preserve fluorescence.
Precautions
Store at 2-8°C. Handle with care to avoid contamination. Use appropriate controls and optimize conditions for each application to ensure specificity and sensitivity.
Validation Images