Goat Anti-Mouse IgG/PE
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.19310-100ul | €200.00 |
| Catalog No | CAB.19310 |
|---|---|
| Product Name | Goat Anti-Mouse IgG/PE |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Mouse IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Mouse IgG/PE is a versatile antibody that can be used in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
Reagents or buffers: Goat Anti-Mouse IgG/PE, phosphate-buffered saline (PBS), mounting medium.
Sample preparation: Fix cells or tissue sections with a suitable fixative, then permeabilize if necessary.
Antibody incubation: Incubate the sample with the primary antibody, followed by the Goat Anti-Mouse IgG/PE at a dilution of 1:100-1000.
Washing or detection: Wash the sample with PBS, then mount with a suitable mounting medium.
Technical notes: Optimize the dilution of the Goat Anti-Mouse IgG/PE for the specific application.
Reagents or buffers: Goat Anti-Mouse IgG/PE, phosphate-buffered saline (PBS), FCM buffer.
Sample preparation: Prepare cell suspensions and block non-specific binding sites.
Antibody incubation: Incubate the cell suspension with the primary antibody, followed by the Goat Anti-Mouse IgG/PE at a dilution of 1:100-1000.
Washing or detection: Wash the cell suspension with FCM buffer, then analyze by flow cytometry.
Technical notes: Optimize the dilution of the Goat Anti-Mouse IgG/PE for the specific application and cell type.
Use assay conditions validated for IF, apply Goat Anti-Mouse IgG/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Mouse IgG/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for each application.
Validation Images