Mouse Anti-Bovine IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.19475-100ul | €200.00 |
| Catalog No | CAB.19475 |
|---|---|
| Product Name | Mouse Anti-Bovine IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Bovine IgG/APC is a highly specific antibody for detecting bovine IgG in various applications.
For IF, the following reagents are required: phosphate-buffered saline (PBS), 4% paraformaldehyde, and a mounting medium with DAPI.
Sample preparation involves fixing cells or tissue sections with 4% paraformaldehyde for 15 minutes, followed by permeabilization with 0.1% Triton X-100 for 10 minutes.
The antibody should be diluted to 1:100-1000 in PBS and incubated with the sample for 1 hour at room temperature.
CABter washing with PBS, a secondary antibody conjugated to a fluorescent dye can be applied if necessary.
Technical notes: Use a negative control without primary antibody to assess background fluorescence.
For FCM, the following reagents are required: PBS, 1% bovine serum albumin (BSA), and a fixative such as 1% paraformaldehyde.
Sample preparation involves washing cells with PBS and resuspending them in PBS with 1% BSA.
The antibody should be diluted to 1:100-1000 in PBS with 1% BSA and incubated with the cells for 30 minutes on ice.
CABter washing with PBS, cells can be fixed with 1% paraformaldehyde and analyzed by flow cytometry.
Technical notes: Use a negative control without primary antibody to set the gate for positive staining.
Both IF and FCM require optimization of antibody dilution and incubation times to achieve the best results.
Use assay conditions validated for IF, apply Mouse Anti-Bovine IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Mouse Anti-Bovine IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 4°C, handle with care, include negative controls, and optimize antibody dilution and incubation times for best results.
Validation Images