Donkey Anti-Goat IgG/Solar Fluor 594

The Donkey Anti-Goat IgG/Solar Fluor 594 is a highly specific secondary antibody conjugated to Solar Fluor 594, suitable for various applications including Immunofluorescence (IF) and Flow Cytometry (FCM).

For IF, the following steps are recommended:

- Reagents or Buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, 0.1% Triton X-100 for permeabilization, and 1% bovine serum albumin (BSA) for blocking.

- Sample Preparation: Fix and permeabilize cells or tissue sections, then block with 1% BSA in PBS.

- Antibody Incubation: Incubate samples with primary goat antibody followed by the Donkey Anti-Goat IgG/Solar Fluor 594 at a dilution of 1:100 to 1:1000 in PBS containing 1% BSA for 1 hour at room temperature.

- Washing or Detection: Wash samples thoroughly with PBS, and detect the signal using a fluorescence microscope.

- Technical Notes: Optimize the dilution of the secondary antibody and the incubation time for best results.

For FCM, follow these guidelines:

- Reagents or Buffers: PBS, 4% paraformaldehyde for fixation, and 0.1% Triton X-100 if intracellular staining is required.

- Sample Preparation: Fix cells with 4% paraformaldehyde, and if necessary, permeabilize with 0.1% Triton X-100.

- Antibody Incubation: Incubate cells with primary goat antibody followed by the Donkey Anti-Goat IgG/Solar Fluor 594 at a dilution of 1:100 to 1:1000 in PBS for 30 minutes to 1 hour on ice.

- Washing or Detection: Wash cells with PBS, and analyze using a flow cytometer.

- Technical Notes: Use appropriate controls, and optimize the antibody dilution and incubation time for optimal signal-to-noise ratio.

Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 594 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;

FCM=1:100-1000.

Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/Solar Fluor 594 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;

FCM=1:100-1000.