Cy5 tagged OVA
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.21184-100ul | €87.50 |
| Catalog No | CAB.21184 |
|---|---|
| Product Name | Cy5 tagged OVA |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | OVA |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Cy5 tagged OVA antibody is a valuable tool for various applications, including Immunofluorescence (IF). Below are the protocols for each validated application.
For IF, the following steps are recommended:
- Reagents or buffers: Cy5 tagged OVA antibody, fluorescent mounting medium, and appropriate wash buffers.
- Sample preparation: Fix and permeabilize cells or tissue sections according to standard protocols.
- Antibody incubation: Incubate samples with the Cy5 tagged OVA antibody at a dilution of 1:100-1000 for 1-2 hours at room temperature or overnight at 4°C.
- Washing or detection: Wash samples thoroughly with wash buffer, then mount with fluorescent mounting medium.
- Technical notes: Optimize antibody dilution and incubation times as needed for specific applications.
Use assay conditions validated for IF, apply Cy5 tagged OVA at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF 1:100-1000..
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for best results.
Validation Images