Anti-CAV1 Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.21304-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.21304-100ul | €437.50 |
| Catalog No | CAB.21304 |
|---|---|
| Product Name | Anti-CAV1 Monoclonal Antibody |
| Isotype | IgG1 |
| Calculated MW | N/A |
| Immunogen | Recombinant protein of human CAV1 |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Human;Mouse;Rat |
| Application | WB;FCM |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.03% Proclin 300. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN，an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.
Protocol / Instructions
The Anti-CAV1 Monoclonal Antibody is a high-quality antibody that can be used for various applications, including Western Blotting (WB) and Flow Cytometry (FCM).
Reagents or buffers: RIPA buffer, SDS-PAGE gel, transfer buffer, blocking buffer, and ECL substrate.
Sample preparation: Prepare cell lysates using RIPA buffer and separate proteins by SDS-PAGE.
Antibody incubation: Incubate the membrane with the Anti-CAV1 Monoclonal Antibody at a suitable dilution in blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash the membrane with PBST and detect the signal using ECL substrate.
Technical notes: The expected band size is around 20-25 kDa.
Reagents or buffers: PBS, FACS buffer, and fixation buffer.
Sample preparation: Prepare single-cell suspensions and fix the cells using fixation buffer.
Antibody incubation: Incubate the cells with the Anti-CAV1 Monoclonal Antibody at a suitable dilution in FACS buffer for 30 minutes at 4°C.
Washing or detection: Wash the cells with FACS buffer and detect the signal using a flow cytometer.
Technical notes: The antibody can be used to detect CAV1 expression on the surface of various cell types.
Use assay conditions validated for FCM, apply Anti-CAV1 Monoclonal Antibody at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Optimize the working dilution empirically using appropriate positive and negative controls.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for each application.
Validation Images