Anti-CD71(APC) Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25T | CAB.21589-25T | €262.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.21589-100T | €637.50 |
| Catalog No | CAB.21589 |
|---|---|
| Product Name | Anti-CD71(APC) Monoclonal Antibody |
| Isotype | IgG1 |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Human |
| Application | FCM |
| Subcellular Location | Cell membrane |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Store at 4℃ in dark.Do not freeze. |
Background
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of CABfinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations，and metabolic activity. A second ligand，the heditary hemochromatosis protein HFE，competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells，including normally cycling in vivo hematopoietic progenitor cells，mitogenically stimulated cells in vitro，some primary tumor cells and most proliferating cells in vitro.
Protocol / Instructions
The Anti-CD71(APC) Monoclonal Antibody is a mouse monoclonal antibody that recognizes the human CD71 antigen. This antibody is recommended for use in flow cytometry (FCM) at a dilution of 5uL/Test.
Reagents or buffers: phosphate-buffered saline (PBS), fetal bovine serum (FBS), and a flow cytometer.
Sample preparation: Prepare a single cell suspension from the sample of interest and wash with PBS.
Antibody incubation: Add 5uL of the Anti-CD71(APC) Monoclonal Antibody to the cell suspension and incubate for 30 minutes at 4°C.
Washing or detection: Wash the cells with PBS and analyze using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time as needed for the specific application.
Use assay conditions validated for FCM, apply Anti-CD71(APC) Monoclonal Antibody at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM 5uL/Test..
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles, and optimize antibody dilution for best results.
Validation Images