Mouse Anti Duck IgY Monoclonal antibody（Light chain）/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.21687-100ul | €87.50 |
| Catalog No | CAB.21687 |
|---|---|
| Product Name | Mouse Anti Duck IgY Monoclonal antibody（Light chain）/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Duck IgY（Light chain） |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti Duck IgY Monoclonal antibody (Light chain)/AP is a versatile tool for detecting Duck IgY light chain in various applications. Below are the protocols for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and AP substrate.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a membrane.
Antibody incubation: Incubate the membrane with the antibody at a dilution of 1:1000-10000 in TBST with 5% skim milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST, then detect using AP substrate.
Technical notes: Optimize the blocking conditions to reduce non-specific binding.
Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, and AP substrate.
Sample preparation: Fix and embed tissue samples, then section and deparaffinize.
Antibody incubation: Incubate the sections with the antibody at a dilution of 1:100-1000 in antibody diluent for 1 hour at room temperature.
Washing or detection: Wash the sections with PBS, then detect using AP substrate.
Technical notes: Perform antigen retrieval to enhance signal.
Reagents or buffers: Methanol, PBS, and AP substrate.
Sample preparation: Fix cells or tissue samples with methanol.
Antibody incubation: Incubate the samples with the antibody at a dilution of 1:100-1000 in antibody diluent for 1 hour at room temperature.
Washing or detection: Wash the samples with PBS, then detect using AP substrate.
Technical notes: Use a fluorescence quencher to reduce background.
Reagents or buffers: Coating buffer, blocking buffer, and AP substrate.
Sample preparation: Coat the plate with antigen and block with blocking buffer.
Antibody incubation: Incubate the plate with the antibody at a dilution of 1:1000-10000 in antibody diluent for 1 hour at room temperature.
Washing or detection: Wash the plate with PBS, then detect using AP substrate.
Technical notes: Optimize the coating and blocking conditions to reduce non-specific binding.
Reagents or buffers: Methanol, PBS, and AP substrate.
Sample preparation: Fix cells or tissue samples with methanol.
Antibody incubation: Incubate the samples with the antibody at a dilution of 1:100-1000 in antibody diluent for 1 hour at room temperature.
Washing or detection: Wash the samples with PBS, then detect using AP substrate.
Technical notes: Use a fluorescence quencher to reduce background.
Precautions
Store at 4°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images