Anti-CD71 Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.21814-1mg | €167.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ug | CAB.21814-100ug | €677.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.21814-100ul | €150.00 |
| Catalog No | CAB.21814 |
|---|---|
| Product Name | Anti-CD71 Monoclonal Antibody |
| Isotype | IgG1 |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Human |
| Application | FCM |
| Subcellular Location | Cell membrane |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Store at 4℃ in dark.Do not freeze. |
Background
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of CABfinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations，and metabolic activity. A second ligand，the heditary hemochromatosis protein HFE，competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells，including normally cycling in vivo hematopoietic progenitor cells，mitogenically stimulated cells in vitro，some primary tumor cells and most proliferating cells in vitro.
Protocol / Instructions
The Anti-CD71 Monoclonal Antibody is a mouse-derived antibody that recognizes the human CD71 antigen, also known as the transferrin receptor. This receptor plays a crucial role in the cellular uptake of iron and is necessary for the development of erythrocytes and the nervous system.
For flow cytometry, prepare a single-cell suspension from the sample of interest. Use a suitable buffer such as PBS with 1% BSA to prevent non-specific binding. Add the Anti-CD71 Monoclonal Antibody at the recommended dilution and incubate for 15-30 minutes at 4°C. Wash the cells with the buffer to remove excess antibody. If using a secondary antibody, incubate for an additional 15-30 minutes at 4°C. Wash the cells again and analyze using a flow cytometer. Technical notes: Use a suitable isotype control to verify the specificity of the antibody.
Use assay conditions validated for FCM, apply Anti-CD71 Monoclonal Antibody at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Optimize the working dilution empirically using appropriate positive and negative controls.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for each application.
Validation Images