Goat anti-Rabbit IgG/Biotin
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.21823-0.1ml | €27.50 |
| Catalog No | CAB.21823 |
|---|---|
| Product Name | Goat anti-Rabbit IgG/Biotin |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | WB;IHC-P;IHC-F;IF;FCM;ELISA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Rabbit IgG/Biotin antibody is a versatile reagent applicable in various immunological techniques.
For Western blotting, use a dilution of 1:2000-20000. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE and transfer to a membrane. Block the membrane with 5% non-fat milk or BSA, then incubate with the Goat anti-Rabbit IgG/Biotin antibody. Wash with TBS-T, and detect using a streptavidin-conjugated enzyme.
For Immunohistochemistry on paraffin sections (IHC-P), use a dilution of 1:200-1000. Deparaffinize and rehydrate tissue sections, then perform antigen retrieval if necessary. Block endogenous peroxidase and biotin, then incubate with the primary rabbit antibody followed by the Goat anti-Rabbit IgG/Biotin. Use a biotin-avidin detection system for visualization.
For Immunohistochemistry on frozen sections (IHC-F), use a dilution of 1:200-1000. Fix and permeabilize tissue sections, then block with an appropriate buffer. Incubate with the primary rabbit antibody, followed by the Goat anti-Rabbit IgG/Biotin. Detect using a fluorescent streptavidin conjugate.
For ELISA, use a dilution of 1:2000-20000. Coat the plate with the antigen, block with an appropriate buffer, then incubate with the primary rabbit antibody. Add the Goat anti-Rabbit IgG/Biotin, followed by a streptavidin-conjugated enzyme. Develop the signal using an appropriate substrate.
For Immunofluorescence (IF), use a dilution of 1:100-1000. Fix and permeabilize cells, then block with an appropriate buffer. Incubate with the primary rabbit antibody, followed by the Goat anti-Rabbit IgG/Biotin. Detect using a fluorescent streptavidin conjugate.
For Flow Cytometry (FCM), use a dilution of 1:100-1000. Fix and permeabilize cells if necessary, then incubate with the primary rabbit antibody. Add the Goat anti-Rabbit IgG/Biotin, followed by a streptavidin-conjugated fluorescent probe. Analyze by flow cytometry.
Technical notes: Optimize the dilution and incubation time for each application to achieve the best results. Use appropriate controls to verify the specificity of the staining or signal.
Precautions
Store at 2-8°C. Handle with care, avoiding freeze-thaw cycles. Use appropriate controls and optimize conditions for each application to minimize non-specific binding.
Validation Images