Anti-ATP1B1 Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.21870-50ul | €437.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.21870-100ul | €730.00 |
| Catalog No | CAB.21870 |
|---|---|
| Product Name | Anti-ATP1B1 Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 35kDa |
| Immunogen | Recombinant protein of human ATP1B1 |
| Public Immunogen Range | 63-303aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Mouse;Human;Rat |
| Application | ELISA;IF/ICC;IHC-P;WB |
| Subcellular Location | Cell membrane Single-pass type II membrane protein |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
Protocol / Instructions
The Anti-ATP1B1 Polyclonal Antibody is a versatile tool for detecting ATP1B1 in various applications. Below are the protocols for each validated application.
Reagents or buffers: Anti-ATP1B1 Polyclonal Antibody, recombinant ATP1B1 protein, ELISA plate, blocking buffer, washing buffer, substrate.
Sample preparation: Coat the ELISA plate with recombinant ATP1B1 protein, block with blocking buffer, and prepare the sample.
Antibody incubation: Incubate the Anti-ATP1B1 Polyclonal Antibody at a recommended dilution of 1:100-1:5000 for 1-2 hours at room temperature.
Washing or detection: Wash the plate with washing buffer and detect using a suitable substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Anti-ATP1B1 Polyclonal Antibody, cell culture, fixation buffer, permeabilization buffer, mounting medium.
Sample preparation: Fix and permeabilize the cells, and prepare the sample.
Antibody incubation: Incubate the Anti-ATP1B1 Polyclonal Antibody at a recommended dilution of 1:50-200 for 1-2 hours at room temperature.
Washing or detection: Wash the cells with washing buffer and detect using a suitable fluorescent secondary antibody.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Anti-ATP1B1 Polyclonal Antibody, paraffin-embedded tissue section, antigen retrieval buffer, blocking buffer, washing buffer, chromogen.
Sample preparation: De-paraffinize and rehydrate the tissue section, and perform antigen retrieval.
Antibody incubation: Incubate the Anti-ATP1B1 Polyclonal Antibody at a recommended dilution of 1:50-200 for 1-2 hours at room temperature.
Washing or detection: Wash the section with washing buffer and detect using a suitable chromogen.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Anti-ATP1B1 Polyclonal Antibody, cell lysate, SDS-PAGE gel, transfer buffer, blocking buffer, washing buffer, substrate.
Sample preparation: Prepare the cell lysate and separate the proteins using SDS-PAGE.
Antibody incubation: Incubate the Anti-ATP1B1 Polyclonal Antibody at a recommended dilution of 1:500-2000 for 1-2 hours at room temperature.
Washing or detection: Wash the membrane with washing buffer and detect using a suitable substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images