Donkey Anti-Guinea Pig IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.22202-100ul | €200.00 |
| Catalog No | CAB.22202 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/APC is a versatile antibody suitable for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: phosphate-buffered saline (PBS), paraformaldehyde (PFA), Triton X-100, and a mounting medium with DAPI.
Sample preparation: Fix cells or tissue sections with 4% PFA, followed by permeabilization with 0.1% Triton X-100.
Antibody incubation: Incubate samples with the Donkey Anti-Guinea Pig IgG/APC at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: Wash samples with PBS and mount with a DAPI-containing medium for nuclei staining.
Technical notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions.
Reagents or buffers: PBS, FBS, and a fixation buffer.
Sample preparation: Prepare cell suspensions and block non-specific binding sites with 1% FBS.
Antibody incubation: Incubate cells with the Donkey Anti-Guinea Pig IgG/APC at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: Wash cells with PBS and analyze using a flow cytometer.
Technical notes: Use a suitable compensation control to account for spectral overlap between the APC fluorophore and other fluorescent dyes.
Use assay conditions validated for IF, apply Donkey Anti-Guinea Pig IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Guinea Pig IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation conditions to minimize non-specific binding and background fluorescence.
Validation Images