Goat anti-Human Fibrinogen/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5ml | CAB.22612-0.5ml | €70.00 |
| Catalog No | CAB.22612 |
|---|---|
| Product Name | Goat anti-Human Fibrinogen/RBITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human Fibrinogen |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Human Fibrinogen/RBITC antibody is designed for use in various applications. Below are the protocols for each validated application.
For IF, the following steps are recommended:
- Reagents or buffers: Use a suitable fixation method such as paraformaldehyde, followed by permeabilization with a detergent like Triton X-100.
- Sample preparation: Prepare your samples according to your specific experimental needs, ensuring they are fixed and permeabilized.
- Antibody incubation: Dilute the Goat anti-Human Fibrinogen/RBITC antibody to 1:50-500 in an appropriate diluent, and incubate with the sample for 1-2 hours at room temperature or overnight at 4°C.
- Washing or detection: Wash the samples thoroughly with a buffer like PBS, and proceed with detection using a fluorescence microscope.
- Technical notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions and the expression level of the target protein.
## Additional Considerations
Given the single validated application of IF for this antibody, the above protocol is tailored to this specific use. For any future applications, similar principles of sample preparation, antibody incubation, and detection will apply, with adjustments made according to the specific requirements of each technique.
Use assay conditions validated for IF, apply Goat anti-Human Fibrinogen/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C. Handle with care, and use appropriate controls. Optimize antibody dilution and incubation times for best results.
Validation Images