Goat Anti-Bovine IgG/Gold 35nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.23544-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.23544-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.23544-100ul | €437.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.23544-100ul(PBS Only) | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.23544-500ul | €42.50 |
| Catalog No | CAB.23544 |
|---|---|
| Product Name | Goat Anti-Bovine IgG/Gold 35nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | GICA;IGS;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 35nm
Protocol / Instructions
The Goat Anti-Bovine IgG/Gold 35nm antibody is designed for use in various immunoassay applications.
For Grid Immunocytochemistry Applications (GICA), start by preparing the sample and grids according to standard protocols.
Reagents or buffers required include the Goat Anti-Bovine IgG/Gold 35nm antibody, phosphate buffer, and distilled water.
Sample preparation involves applying the sample to the grid and allowing it to adhere.
Antibody incubation is performed by applying the Goat Anti-Bovine IgG/Gold 35nm antibody at a dilution of 1:20-200 in phosphate buffer for 30 minutes to 1 hour.
Washing is done with phosphate buffer, followed by distilled water.
Detection is achieved through the visualization of the gold particles using an electron microscope.
Technical notes: Optimize the antibody dilution and incubation time for best results.
For Immunogold Silver Staining (IGS), begin by preparing the sample and sections according to standard protocols.
Reagents or buffers required include the Goat Anti-Bovine IgG/Gold 35nm antibody, phosphate buffer, silver enhancement solution, and distilled water.
Sample preparation involves applying the sample to the section and allowing it to adhere.
Antibody incubation is performed by applying the Goat Anti-Bovine IgG/Gold 35nm antibody at a dilution of 1:20-200 in phosphate buffer for 30 minutes to 1 hour.
Washing is done with phosphate buffer, followed by distilled water.
Detection is achieved through the silver enhancement of the gold particles.
Technical notes: Optimize the silver enhancement time for best results.
For Immunoelectron Microscopy (IEM), start by preparing the sample and grids according to standard protocols.
Reagents or buffers required include the Goat Anti-Bovine IgG/Gold 35nm antibody, phosphate buffer, and distilled water.
Sample preparation involves applying the sample to the grid and allowing it to adhere.
Antibody incubation is performed by applying the Goat Anti-Bovine IgG/Gold 35nm antibody at a dilution of 1:20-200 in phosphate buffer for 30 minutes to 1 hour.
Washing is done with phosphate buffer, followed by distilled water.
Detection is achieved through the visualization of the gold particles using an electron microscope.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images