Goat Anti-Mouse IgM/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.23837-100ul | €200.00 |
| Catalog No | CAB.23837 |
|---|---|
| Product Name | Goat Anti-Mouse IgM/APC |
| Isotype | N/A |
| Calculated MW | 900kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Mouse IgM |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Mouse IgM/APC is a versatile antibody that can be used in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
Reagents or buffers: Goat Anti-Mouse IgM/APC, phosphate-buffered saline (PBS), paraformaldehyde (PFA), and a mounting medium.
Sample preparation: Fix cells or tissue sections with 4% PFA, followed by permeabilization with 0.1% Triton X-100 if necessary.
Antibody incubation: Incubate samples with the Goat Anti-Mouse IgM/APC at a dilution of 1:100-1000 in PBS for 1 hour at room temperature or overnight at 4°C.
Washing or detection: Wash samples with PBS, and then proceed with fluorescence microscopy.
Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions.
Reagents or buffers: Goat Anti-Mouse IgM/APC, phosphate-buffered saline (PBS), and a flow cytometry buffer.
Sample preparation: Prepare single-cell suspensions from tissues or cells, and wash with PBS.
Antibody incubation: Incubate cells with the Goat Anti-Mouse IgM/APC at a dilution of 1:100-1000 in PBS for 30 minutes to 1 hour on ice.
Washing or detection: Wash cells with PBS, and then analyze using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions, and consider using a viability dye to exclude dead cells.
Use assay conditions validated for IF, apply Goat Anti-Mouse IgM/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Mouse IgM/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for specific applications.
Validation Images