Donkey Anti-Rabbit IgG/Bio
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.23859-100ul | €27.50 |
| Catalog No | CAB.23859 |
|---|---|
| Product Name | Donkey Anti-Rabbit IgG/Bio |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Rabbit IgG/Bio is a versatile antibody that can be used in various applications.
For Western Blotting, use the antibody at a dilution of 1:1000-10000. The reagents required include the antibody, blocking buffer, and washing buffer. Prepare the sample by separating the proteins using SDS-PAGE and transferring them to a membrane. Block the membrane with blocking buffer and then incubate with the antibody. Wash the membrane with washing buffer and detect using a suitable detection method. Technical notes: Optimize the blocking and washing steps to minimize background noise.
For ELISA, use the antibody at a dilution of 1:1000-10000. The reagents required include the antibody, coating buffer, blocking buffer, and substrate. Prepare the sample by coating the plate with the antigen and blocking with blocking buffer. Incubate with the antibody and then add the substrate to detect. Technical notes: Use a suitable negative control to optimize the assay.
For IHC-P, use the antibody at a dilution of 1:100-1000. The reagents required include the antibody, antigen retrieval buffer, and chromogen. Prepare the sample by sectioning the tissue and performing antigen retrieval. Incubate with the antibody and then add the chromogen to detect. Technical notes: Optimize the antigen retrieval step to enhance signal.
For IHC-F, use the antibody at a dilution of 1:100-1000. The reagents required include the antibody, permeabilization buffer, and fluorophore. Prepare the sample by sectioning the tissue and permeabilizing with permeabilization buffer. Incubate with the antibody and then add the fluorophore to detect. Technical notes: Use a suitable mounting medium to preserve the signal.
For IF, use the antibody at a dilution of 1:100-1000. The reagents required include the antibody, permeabilization buffer, and fluorophore. Prepare the sample by fixing and permeabilizing with permeabilization buffer. Incubate with the antibody and then add the fluorophore to detect. Technical notes: Optimize the fixation step to preserve the signal.
Precautions
Store at 2-8°C. Handle with care. Use suitable controls and optimize assay conditions to minimize background and enhance signal.
Validation Images