Donkey Anti-Goat IgG/Gold 20nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.24405-500ul | €42.50 |
| Catalog No | CAB.24405 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Gold 20nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IGS;GICA;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 20nm
Protocol / Instructions
The Donkey Anti-Goat IgG/Gold 20nm is a versatile antibody conjugate suitable for various applications including Immunogold Silver Staining (IGS), Immunogold Chromogenic Amplification (GICA), and Immunoelectron Microscopy (IEM).
Reagents or buffers: Donkey Anti-Goat IgG/Gold 20nm, silver enhancement solution, and PBS.
Sample preparation: Prepare tissue sections or cell samples on glass slides.
Antibody incubation: Incubate samples with primary goat antibody followed by Donkey Anti-Goat IgG/Gold 20nm at a dilution of 1:20-200.
Washing or detection: Wash samples with PBS and detect using silver enhancement solution.
Technical notes: Optimize silver enhancement time to avoid over-staining.
Reagents or buffers: Donkey Anti-Goat IgG/Gold 20nm, chromogenic substrate, and PBS.
Sample preparation: Prepare tissue sections or cell samples on glass slides.
Antibody incubation: Incubate samples with primary goat antibody followed by Donkey Anti-Goat IgG/Gold 20nm at a dilution of 1:20-200.
Washing or detection: Wash samples with PBS and detect using chromogenic substrate.
Technical notes: Choose the appropriate chromogenic substrate for the desired colorimetric reaction.
Reagents or buffers: Donkey Anti-Goat IgG/Gold 20nm and PBS.
Sample preparation: Prepare ultrathin tissue sections on grids.
Antibody incubation: Incubate samples with primary goat antibody followed by Donkey Anti-Goat IgG/Gold 20nm at a dilution of 1:20-200.
Washing or detection: Wash samples with PBS and view using a transmission electron microscope.
Technical notes: Optimize sample preparation and antibody incubation conditions for optimal labeling.
Use assay conditions validated for IGS, apply Donkey Anti-Goat IgG/Gold 20nm at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IEM=1:20-200;
IGS=1:20-200;
GICA=1:20-200.
Use assay conditions validated for GICA, apply Donkey Anti-Goat IgG/Gold 20nm at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IEM=1:20-200;
IGS=1:20-200;
GICA=1:20-200.
Use assay conditions validated for IEM, apply Donkey Anti-Goat IgG/Gold 20nm at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IEM=1:20-200;
IGS=1:20-200;
GICA=1:20-200.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions for each application.
Validation Images