Donkey Anti-Goat IgG/Solar Fluor 640
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.25340-100ul | €87.50 |
| Catalog No | CAB.25340 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 640 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 640 is a secondary antibody used for detecting goat IgG primary antibodies.
Reagents or buffers: phosphate-buffered saline (PBS), secondary antibody, mounting medium.
Sample preparation: fix and permeabilize cells or tissue sections, block with a blocking buffer.
Antibody incubation: incubate samples with the primary antibody, followed by the Donkey Anti-Goat IgG/Solar Fluor 640 secondary antibody at a dilution of 1:50-500.
Washing or detection: wash samples with PBS, then detect the signal using a fluorescence microscope.
Technical notes: optimize the dilution of the secondary antibody for the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 640 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images