Goat Anti-Mouse IgM/Bio
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.25976-100ul | €100.00 |
| Catalog No | CAB.25976 |
|---|---|
| Product Name | Goat Anti-Mouse IgM/Bio |
| Isotype | N/A |
| Calculated MW | 900kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Mouse IgM |
| Application | WB;IHC-P;IHC-F;IF;FCM;ELISA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Mouse IgM/Bio antibody is a versatile tool for detecting Mouse IgM in various applications.
For Western Blotting, dilute the antibody to 1:1000-10000 in the recommended buffer. Prepare samples by lysing cells or tissues, then separate proteins by gel electrophoresis and transfer to a membrane. Incubate the membrane with the diluted antibody, followed by washing and detection using a suitable method. Technical notes: Optimize the dilution and detection method for the specific sample and experimental setup.
For Immunohistochemistry on paraffin-embedded tissues, dilute the antibody to 1:100-500. Prepare tissue sections, then incubate with the diluted antibody. Perform washing and detection steps according to the chosen protocol. Technical notes: Antigen retrieval may be necessary for optimal staining.
For Immunohistochemistry on frozen tissues, dilute the antibody to 1:100-1000. Prepare tissue sections, fix, and permeabilize as necessary. Incubate with the diluted antibody, followed by washing and detection. Technical notes: Optimization of fixation and permeabilization steps may be required.
For Immunofluorescence, dilute the antibody to 1:100-1000. Prepare cell or tissue samples, fix, and permeabilize as necessary. Incubate with the diluted antibody, then wash and detect using a fluorescent secondary antibody. Technical notes: Choose a secondary antibody that is compatible with the Goat Anti-Mouse IgM/Bio.
For Flow Cytometry, dilute the antibody to 1:100-1000. Prepare cell suspensions, and incubate with the diluted antibody. Perform washing and detection steps according to the flow cytometer's specifications. Technical notes: Compensation and gating strategies should be optimized for the specific experiment.
For Enzyme-Linked Immunosorbent Assay, dilute the antibody to 1:1000-10000. Prepare the ELISA plate by coating with the antigen, then incubate with the diluted antibody. Perform washing, detection, and quantification steps according to the ELISA kit's instructions. Technical notes: Optimize the coating and incubation conditions for the specific antigen and antibody combination.
The choice of dilution and detection method may vary depending on the specific application and sample type. It is essential to optimize these parameters for each experiment to achieve the best results.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution and detection methods for each application to ensure specificity and sensitivity.
Validation Images