Donkey Anti-Goat IgG/Cy7
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.26061-100ul | €67.50 |
| Catalog No | CAB.26061 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Cy7 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Cy7 antibody is a highly specific reagent designed for various applications. Below are the protocols for the validated application.
For IF, the following steps are recommended:
- Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, and a suitable mounting medium.
- Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize if necessary, and block with a suitable blocking buffer.
- Antibody incubation: Incubate the sample with the Donkey Anti-Goat IgG/Cy7 antibody at a dilution of 1:50-500 in PBS for 1-2 hours at room temperature or overnight at 4°C.
- Washing or detection: Wash the sample thoroughly with PBS, and proceed with fluorescence microscopy. The Cy7 fluorophore has an excitation maximum at approximately 743 nm and an emission maximum at approximately 767 nm.
- Technical notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions and the intensity of the fluorescence signal.
## General Considerations
Regardless of the application, it is crucial to handle the antibody with care, avoiding exposure to light and moisture, which can degrade the fluorophore.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Cy7 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, protect from light, and handle with care to avoid contamination and degradation.
Validation Images