Goat anti-Chicken IgG/Gold 10nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.27041-500ul | €75.00 |
| Catalog No | CAB.27041 |
|---|---|
| Product Name | Goat anti-Chicken IgG/Gold 10nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Chicken IgG |
| Application | GICA;IGS;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 10nm
Protocol / Instructions
The Goat anti-Chicken IgG/Gold 10nm antibody is designed for various immunogold labeling applications.
For Grid Immunocytochemistry Applications (GICA), start by preparing the sample and sectioning it into suitable sizes.
Use a blocking buffer to prevent non-specific binding, then incubate the sample with the Goat anti-Chicken IgG/Gold 10nm antibody at a dilution of 1:20-200.
CABter incubation, wash the sample with a washing buffer to remove excess antibodies.
Technical notes: Ensure proper fixation and washing to minimize background noise.
For Immunogold Silver Staining (IGS), prepare the sample and apply the primary antibody.
Then, incubate the sample with the Goat anti-Chicken IgG/Gold 10nm antibody at a dilution of 1:20-200.
Use a silver enhancement reagent to amplify the signal.
Sample preparation is crucial for optimal results, and the choice of silver enhancement reagent may CABfect the outcome.
For Immunoelectron Microscopy (IEM), prepare the sample by fixation and sectioning.
Incubate the sample with the primary antibody, followed by the Goat anti-Chicken IgG/Gold 10nm antibody at a dilution of 1:20-200.
Use a washing buffer to remove excess antibodies, and then analyze the sample using an electron microscope.
General considerations: Optimize the antibody dilution for each specific application to achieve the best results.
The particle size of 10nm allows for high-resolution imaging and detection.
Precautions
Store at 2-8°C. Handle with care, and avoid freeze-thaw cycles. Optimize antibody dilution and washing conditions to minimize background and ensure specific binding.
Validation Images