Goat anti-Human Serum (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.27066-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.27066-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.27066-5mL | €1125.00 |
| Catalog No | CAB.27066 |
|---|---|
| Product Name | Goat anti-Human Serum (Immune serum) |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | Human Serum |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Antisera against whole serums are obtained by immunizing host animals with whole serum. This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-Human Serum (Immune serum) is a versatile antibody that can be used in various applications.
For ELISA, the recommended dilution is 1:5000-50000. The reagents required include the antigen, antibody, and enzyme conjugate. Sample preparation involves coating the antigen onto the plate, followed by blocking and washing. The antibody incubation is typically 1-2 hours at room temperature. Washing and detection are then performed using the enzyme conjugate and substrate. Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the sample, antibody, and membrane. Sample preparation involves separating the proteins by SDS-PAGE and transferring them onto a membrane. The antibody incubation is typically overnight at 4°C. Washing and detection are then performed using the secondary antibody and substrate. Technical notes: Use a suitable blocking buffer to reduce non-specific binding.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the antigen and antibody. Sample preparation involves preparing the agar plate and adding the antigen and antibody. The antibody incubation is typically 24-48 hours at room temperature. Technical notes: Optimize the concentration of the antigen and antibody to obtain a clear precipitin line.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize conditions for each application to minimize non-specific binding.
Validation Images