Goat Anti-Human C3/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5ml | CAB.27082-0.5ml | €55.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.27082-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.27082-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.27082-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.27082-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.27082 |
|---|---|
| Product Name | Goat Anti-Human C3/FITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human C3 |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human C3/FITC antibody is designed for use in various applications. Below are the protocols for the validated application.
For IF, the following reagents and buffers are required: phosphate-buffered saline (PBS), a blocking buffer such as 5% normal serum or BSA, and a mounting medium with DAPI for nuclear staining. Sample preparation involves fixing cells or tissue sections with a suitable fixative, followed by permeabilization if necessary. The Goat Anti-Human C3/FITC antibody should be diluted to 1:5-10 in the blocking buffer and incubated with the sample for 1-2 hours at room temperature or overnight at 4°C. CABter washing with PBS, the sample can be mounted and visualized under a fluorescence microscope. Technical notes: the use of a FITC filter is required for detection, and the sample should be protected from light to prevent photobleaching.
Use assay conditions validated for IF, apply Goat Anti-Human C3/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:5-10.
Precautions
Store at 2-8°C, handle with care, optimize antibody dilution, and include proper controls for each experiment.
Validation Images