Anti-A4GNT Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.27437-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.27437-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.27437-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.27437-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.27437 |
|---|---|
| Product Name | Anti-A4GNT Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 39kDa |
| Immunogen | Recombinant protein of human A4GNT |
| Public Immunogen Range | 27-340aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | N/A |
| Application | IHC-P |
| Subcellular Location | Cytoplasm |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
Alpha-1,4-N-Acetylglucosaminyltransferase 4, also known as A4GNT is a 45 kDa glycoprotein, member of the glycosyltransferase 32 family of proteins. A4GNT catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. A4GNT is a single-pass type II membrane protein associated with the Golgi apparatus and contains the conserved DXD motif involved in catalytic activity. It is expressed in stomach and pancreas, as well as in gastric cancer cells. A4GNT is not expressed in peripheral blood cells, making it a putative biomarker for pancreatic cancer. A4GNT and Mucin 6 expression is upregulated in the gastric mucosa of H. pylori infected patients. Lumenal domain of human A4GNT shares 72% aa sequence identity with mouse and rat A4GNT.
Protocol / Instructions
The Anti-A4GNT Polyclonal Antibody is a rabbit-derived antibody raised against a recombinant protein of human A4GNT. This antibody is recommended for use in Immunohistochemistry-Paraffin sections (IHC-P) at a dilution of 1:50-200.
Reagents or buffers required include the Anti-A4GNT Polyclonal Antibody, paraffin-embedded tissue sections, xylene, ethanol, antigen retrieval buffer, and a detection system such as DAB or alkaline phosphatase.
Sample preparation involves deparaffinization of tissue sections using xylene and ethanol, followed by antigen retrieval using a suitable buffer.
Antibody incubation is performed by applying the Anti-A4GNT Polyclonal Antibody at the recommended dilution and incubating for a suitable duration, typically 1-2 hours at room temperature or overnight at 4°C.
Washing is done using a buffer such as PBS or TBS, and detection is achieved using a suitable detection system.
Technical notes: Optimize the antibody dilution and incubation time for best results. Use a suitable negative control to verify the specificity of the staining.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images
