Donkey Anti-Rabbit IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.27738-100ul | €27.50 |
| Catalog No | CAB.27738 |
|---|---|
| Product Name | Donkey Anti-Rabbit IgG/RBITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Rabbit IgG/RBITC is a secondary antibody used for detecting rabbit primary antibodies. It is conjugated to Rhodamine B Isothiocyanate (RBITC), which fluoresces at 550 nm.
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde (PFA) for fixation, 0.1% Triton X-100 for permeabilization.
Sample preparation: Fix cells or tissue sections with 4% PFA for 15-20 minutes, then permeabilize with 0.1% Triton X-100 for 10-15 minutes.
Antibody incubation: Incubate the sample with the primary rabbit antibody, followed by incubation with the Donkey Anti-Rabbit IgG/RBITC at a dilution of 1:50-500 for 1-2 hours at room temperature.
Washing or detection: Wash the sample thoroughly with PBS, then visualize the fluorescence using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for the specific application. Use appropriate controls, such as omitting the primary antibody, to verify the specificity of the staining.
Use assay conditions validated for IF, apply Donkey Anti-Rabbit IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for specific applications to avoid non-specific binding.
Validation Images