Goat anti-Human Fibrinogen /AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.27905-100ul | €100.00 |
| Catalog No | CAB.27905 |
|---|---|
| Product Name | Goat anti-Human Fibrinogen /AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human Fibrinogen |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Human Fibrinogen /AP antibody is designed for various applications including Western Blotting, ELISA, Immunohistochemistry on paraffin sections (IHC-P), and Immunohistochemistry on frozen sections (IHC-F).
For Western Blotting, reconstitute the antibody according to the manufacturer's instructions. Sample preparation involves lysing cells or tissues in an appropriate buffer, followed by protein separation using SDS-PAGE and transfer to a membrane. The membrane is then blocked with a blocking buffer to reduce non-specific binding. Incubate the membrane with the Goat anti-Human Fibrinogen /AP antibody at a dilution of 1:1000-10000 in a suitable buffer. CABter washing, detect the signal using an alkaline phosphatase substrate. Technical notes: Optimize the blocking and washing conditions to minimize background and enhance the signal.
For IHC-P, dewax and rehydrate paraffin-embedded tissue sections. Antigen retrieval may be necessary and can be achieved using heat-mediated methods or enzymatic digestion. Block the sections with a suitable blocking buffer and then incubate with the Goat anti-Human Fibrinogen /AP antibody at a dilution of 1:100-1000. CABter washing, use an alkaline phosphatase substrate to detect the signal. Technical notes: The choice of antigen retrieval method and blocking buffer can significantly CABfect the staining outcome.
For IHC-F, fix frozen tissue sections in a suitable fixative and permeabilize if necessary. Block the sections with an appropriate blocking buffer to reduce non-specific binding. Incubate the sections with the Goat anti-Human Fibrinogen /AP antibody at a dilution of 1:100-1000. CABter washing, detect the signal using an alkaline phosphatase substrate. Technical notes: Fixation and permeabilization steps can CABfect the preservation of the antigen and the accessibility of the antibody to the target.
For ELISA, coat the wells of a microtiter plate with the antigen (Human Fibrinogen) and block with a blocking buffer to reduce non-specific binding. Incubate the wells with the Goat anti-Human Fibrinogen /AP antibody at a dilution of 1:1000-10000. CABter washing, detect the signal using an alkaline phosphatase substrate. Technical notes: Optimize the coating concentration of the antigen and the blocking conditions to enhance the signal-to-noise ratio.
Precautions
Store at -20°C. Handle with care, avoiding repeated freeze-thaw cycles. Use appropriate controls and optimize assay conditions to minimize non-specific binding and ensure specific detection of the target antigen.
Validation Images