Anti-CD71(FITC) Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25T | CAB.28278-25T | €220.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.28278-100T | €512.50 |
| Catalog No | CAB.28278 |
|---|---|
| Product Name | Anti-CD71(FITC) Monoclonal Antibody |
| Isotype | IgG2a |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Rat |
| Clone Type | Monoclonal Antibody |
| Reactivity | Mouse |
| Application | FCM |
| Subcellular Location | basolateral plasma membrane blood microparticle cell surface clathrin-coated pit cytoplasmic vesicle early endosome endosome external side of plasma membrane extracellular exosome extracellular region extracellular space extracellular vesicle melanosome mitochondrion nucleus perinuclear region of cytoplasm plasma membrane recycling endosome |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ in dark.Do not freeze. |
Background
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
Protocol / Instructions
The Anti-CD71(FITC) Monoclonal Antibody is a rat monoclonal antibody that recognizes the mouse CD71 antigen.
Reagents or buffers: phosphate-buffered saline (PBS), FITC-conjugated Anti-CD71 antibody, and a flow cytometer.
Sample preparation: Prepare a single-cell suspension from the tissue of interest and wash with PBS.
Antibody incubation: Add 5uL of the Anti-CD71(FITC) antibody to the cell suspension and incubate for 30 minutes at 4°C.
Washing or detection: Wash the cells with PBS and analyze using a flow cytometer.
Technical notes: Optimize the antibody concentration and incubation time for specific applications.
The Anti-CD71(FITC) Monoclonal Antibody is recommended for use at a dilution of 5uL/Test for FCM.
The antibody is specific for the CD71 antigen, which plays a crucial role in cellular iron uptake and is necessary for erythropoiesis and neurologic development.
Use assay conditions validated for FCM, apply Anti-CD71(FITC) Monoclonal Antibody at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM 5uL/Test..
Precautions
Store at 2-8°C, handle with care, and optimize antibody concentration and incubation time for specific applications.
Validation Images