Anti-AADAC Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.28292-50ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.28292-100ul | €335.00 |
| Catalog No | CAB.28292 |
|---|---|
| Product Name | Anti-AADAC Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 46kDa |
| Immunogen | Recombinant protein of human AADAC |
| Public Immunogen Range | 170-399aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Mouse;Human |
| Application | ELISA;WB |
| Subcellular Location | Endoplasmic reticulum membrane Microsome membrane Single-pass type II membrane protein |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens
Protocol / Instructions
The Anti-AADAC Polyclonal Antibody is a rabbit-derived antibody that recognizes human and mouse AADAC. For optimal results, follow the protocols below for each validated application.
Reagents or buffers: Anti-AADAC Polyclonal Antibody, recombinant AADAC protein, ELISA plates, blocking buffer, washing buffer, substrate.
Sample preparation: Coat ELISA plates with recombinant AADAC protein, block with blocking buffer.
Antibody incubation: Incubate with Anti-AADAC Polyclonal Antibody at a dilution of 1:1000 for 1 hour at room temperature.
Washing or detection: Wash with washing buffer, detect with substrate.
Technical notes: Optimize antibody dilution and incubation time for best results.
Reagents or buffers: Anti-AADAC Polyclonal Antibody, sample lysates, SDS-PAGE gel, transfer buffer, blocking buffer, washing buffer.
Sample preparation: Prepare sample lysates, separate proteins by SDS-PAGE, transfer to membrane.
Antibody incubation: Incubate with Anti-AADAC Polyclonal Antibody at a dilution of 1:500-2000 overnight at 4°C.
Washing or detection: Wash with washing buffer, detect with chemiluminescent substrate.
Technical notes: Use fresh lysates and optimize blocking conditions for best results.
Precautions
Store at -20°C, handle with gloves, use proper controls, optimize antibody dilution and incubation time for best results.
Validation Images