Donkey Anti-Goat IgG/Cy5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.28356-100ul | €67.50 |
| Catalog No | CAB.28356 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Cy5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Cy5 is a highly specific antibody conjugate designed for various applications, including Immunofluorescence (IF).
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde (PFA) for fixation, 0.1% Triton X-100 for permeabilization, and a blocking buffer (e.g., 5% normal donkey serum in PBS).
Sample preparation: Fix cells or tissue sections with 4% PFA, permeabilize with 0.1% Triton X-100, and block with a blocking buffer.
Antibody incubation: Dilute the Donkey Anti-Goat IgG/Cy5 in the recommended dilution range of 1:50-500 in a blocking buffer and incubate for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples thoroughly with PBS CABter antibody incubation. Since the antibody is conjugated to Cy5, a fluorescent dye, it can be directly visualized under a fluorescence microscope with appropriate filters.
Technical notes: Optimize the dilution of the antibody for specific applications and ensure proper controls are included to verify specificity.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Cy5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 4°C, handle with care, include proper controls, and optimize antibody dilution for best results.
Validation Images